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KMID : 0043320100330020285
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Archives of Pharmacal Research
2010 Volume.33 No. 2 p.285 ~ p.291
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Interferon-γ Enhances the Apoptosis of Macrophages Under Trophic Stress through Activation of p53 and the JAK1 Pathway
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Pyo Suhk-Neung
Cho Seong-Jun
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Abstract
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Apoptosis has been implicated as an important mediator of immunosuppression associated with a depleted nutritional state. Since a number of cytokines are likely to influence the rate of apoptosis, we determined the effect of interferon-gamma (IFN-γ) on apoptosis of macrophages in serum/amino acid deprived cell culture conditions. IFN-γ further increased the rate of apoptosis compared to trophically stressed macrophages. In addition, we showed that enhancement of apoptosis by IFN-γ is caused by a decrease in mitochondrial membrane potential due to upregulation of p53 and Bax and down-regulation of Bcl-xL. Moreover, we found that IFN-γ increases caspase-3 activity that had been induced by serum/amino acid depletion, and that JAK1 signaling is involved in capase-3 activation and in the enhancement of apoptosis by IFN-γ.
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KEYWORD
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IFN-γ, Apoptosis, p53, JAK1, Macrophages
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